Trichinella spiralis in a cougar (Puma concolor) hunted by poachers in Chile

Abstract Trichinellosis is a zoonosis caused by nematodes of the Trichinella genus, which 10 species have been described. Species identification when larvae found only possible using molecular tools. Meat from pigs and game animals not subjected to veterinary inspection are main sources human infections. The hunting native carnivores prohibited in Chile due conservation issues fact that those favor pest control. illegal cougar (Puma concolor) occurred September 2020. Herein, larvae, analyzing nuclear (expansion segment V) mitochondrial (cytochrome C oxidase subunit I) sequences Both amplification expansion V region phylogenetic analysis fragment cytochrome c-oxidase I sequence confirmed belonged T. spiralis. case described herein represents first evidence protected mammal infected with Chile, highlighting ‘One Health’ perspective face this disease rural-sylvatic interphase.